Bookcover of Biotransformation of 1-Phenylpyrazole and 1-Phenylpyrrole by NDO
Booktitle:

Biotransformation of 1-Phenylpyrazole and 1-Phenylpyrrole by NDO

Naphthalene Dioxygenase

LAP LAMBERT Academic Publishing (2011-10-10 )

Books loader

Omni badge eligible for voucher
ISBN-13:

978-3-8465-2640-8

ISBN-10:
3846526401
EAN:
9783846526408
Book language:
English
Blurb/Shorttext:
The naphthalene dioxygenase (NDO) from Pseudomonas sp. Strain NCIB 9816-4 is a multicomponent enzyme system that carries out the initial step in the degradation of naphthalene. This enzyme has a broad substrate range and catalyzes several types of reactions including cis-hydroxylation, monooxygenation, and desaturation. In this study 1-phenylpyrazole and 1-phenylpyrrole were tested for biotransformation by NDO. HPLC analysis showed two metabolites from each substrate were produced by NDO. LC/MS analysis indicated the molecular weight of metabolites M1 and M2 of 1-phenylpyrazole were increased by 16 and 34 respectively which could be assumed as monooxygenation and dioxygenation reaction. On the other hand, HPLC and LC/MS analyses of metabolites M1 and M2 from 1-phenylpyrrole showed increased molecular weight of 16 and 32, as compared to the parent compounds, proposing addition of one and two atoms of oxygen to substrate, respectively. The structures of the metabolites were confirmed by 1HNMR and 13CNMR analysis.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
http://www.lap-publishing.com/
By (author) :
Cung Nawl Thawng, Hor-Gil Hur
Number of pages:
80
Published on:
2011-10-10
Stock:
Available
Category:
Microbiology
Price:
4804.51 руб
Keywords:
Biotransformation

Books loader

Newsletter

Wire Transfer

  0 products in the shopping cart
Edit cart
Loading frontend
LOADING